Application

Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.

General description

Useful as an ionizing solvent. Absorbance (0.5 M): ≤0.08 at 260 nm; ≤0.03 at 280 nm. Adenine deaminase inhibitor. Used in DNA stripping during genetic fingerprinting techniques. Supplied as deionized.

Deionized. Adenine deaminase inhibitor. Used in DNA stripping during genetic fingerprinting techniques.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Packaging

1 l in Plastic ampoule

100 ml in Plastic ampoule

Warning

Toxicity: Carcinogenic / Teratogenic (D)

SPECIFICATIONS

autoignition temp.	932 °F
bp	210 °C (lit.)
cation traces	heavy metals (as Pb): ≤0.0001%
density	1.134 g/mL at 25 °C (lit.)
foreign activity	DNases, proteases, RNases, none detected
form	liquid
InChI key	ZHNUHDYFZUAESO-UHFFFAOYSA-N
InChI	1S/CH3NO/c2-1-3/h1H,(H2,2,3)
manufacturer/tradename	Calbiochem^®
mp	2-3 °C (lit.)
pH	8-10 (20 °C, 200 g/L)
Quality Level	100
refractive index	n20/D 1.447 (lit.)
SMILES string	NC=O
solubility	water: 20 mg/mL (~0.5 M)
storage condition	OK to freeze
storage temp.	10-30°C
technique(s)	hybridization: suitable
vapor density	1.55 (vs air)
vapor pressure	30 mmHg ( 129 °C), 0.08 mmHg ( 20 °C)